Harvard University · BiochemistryBuratowski Transcription Lab
Stephen Buratowski · Cambridge, Massachusetts
Research Focus
Stephen Buratowski's lab at Harvard Medical School focuses on understanding the intricate processes of gene expression by examining how RNA polymerase II, an enzyme responsible for transcribing DNA into RNA, is regulated. The lab investigates the role of various protein modifications and interactions during transcription, which has significant implications for diseases such as cancer and viral infections like HIV. By utilizing cutting-edge techniques like mass spectrometry and single-molecule microscopy, they aim to uncover dynamic events that govern gene expression, potentially leading to new therapeutic strategies.
gene expressionRNA processingtranscription regulationmass spectrometrysingle-molecule microscopyHIVchromatinphosphorylationnucleosomes
Research Aims
3AIM 01Analyze phosphorylation dynamics of RNA polymerase II using mass spectrometry.
AIM 02Investigate the function of transcription elongation factors with long linker domains.
AIM 03Visualize transcription elongation processes in real time using single-molecule techniques.
Skills the Lab Values
mass spectrometrysingle-molecule microscopyRNA extractiongel electrophoresischromatographycell cultureprotein purificationtranscription assaysWestern blotting
Output against the department
Publications195top 44% of dept.174th of 402
this lab
most publishedleast published
Citations23ktop 17% of dept.67th of 402
this lab
most citedleast cited
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