Johns Hopkins University · BiologyWu Chromatin Lab
Carl Wu · Baltimore, Maryland
Research Focus
Dr. Carl Wu's lab at Johns Hopkins University focuses on understanding how genes are expressed in living cells by studying the processes that regulate chromatin, the structure that organizes DNA. They use advanced techniques to visualize how proteins interact with DNA and regulate gene activity, particularly under stress conditions like heat. The lab aims to uncover the precise timing and mechanics of these processes to enhance our understanding of gene regulation and its implications for various biological functions.
Chromatin remodelingGene expressionTranscriptionHeat stressSingle-molecule imagingEpigeneticsYeast molecular geneticsNucleosome dynamicsProtein-DNA interactionsBiochemical assays
Research Aims
5AIM 01Investigate the kinetics of chromatin remodeling during transcription.
AIM 02Explore how heat stress affects gene expression through chromatin architecture.
AIM 03Develop new biochemical assays to analyze chromatin accessibility.
AIM 04Identify key intermediates in the transcription process on chromatin.
AIM 05Study the collaboration between transcription factors and chromatin remodelers.
Skills the Lab Values
Single-molecule imagingChromatin immunoprecipitationLive-cell imagingFluorescent microscopyBiochemical assaysPCRYeast geneticsNucleosome remodeling assaysCell cultureData analysis with Python
Output against the department
Publications247top 24% of dept.252nd of 1,033
this lab
most publishedleast published
Citations26ktop 14% of dept.145th of 1,033
this lab
most citedleast cited
Career totals from OpenAlex, compared against labs that share a department. A department spans everything from one paper to tens of thousands, so bar heights are on a log scale — otherwise the largest lab would flatten every other bar to a line.