University of California-San Francisco · Microbiology & ImmunologyBondy-Denomy Phage Lab
Joseph Bondy-Denomy · San Francisco, California
Research Focus
The research lab led by Joseph Bondy-Denomy at UCSF focuses on understanding how bacteria resist infection by bacteriophages, which are viruses that specifically target bacteria. By employing advanced genetic and proteomic techniques, the lab aims to identify bacterial vulnerabilities that could be exploited to improve phage therapy, especially in the context of antibiotic resistance. This work is particularly relevant in the fight against antibiotic-resistant infections, as it seeks to develop new therapeutic strategies that harness the power of these viral predators.
bacteriophagesantibiotic resistancePseudomonas aeruginosaCRISPRprotein-protein interactionsgene knockdownproteomicsphage therapybacterial immune systems
Research Aims
5AIM 01Investigate the role of essential genes in limiting phage replication in bacteria
AIM 02Identify non-essential genes that limit phage replication in strain-dependent manners
AIM 03Map phage-host protein-protein interactions using proteomics
AIM 04Validate important factors identified from genetic and proteomic assays
AIM 05Explore the potential of phage accessory genes to enhance phage therapeutics.
Skills the Lab Values
CRISPR transcriptional interference (CRISPRi)protein-protein interaction mappingmass spectrometrygenetic engineeringcell fractionationbacterial culture techniquesdata analysis using bioinformaticsaffinity purificationphage replication assays
Output against the department
Publications130top 50% of dept.244th of 486
this lab
most publishedleast published
Citations8.9ktop 41% of dept.198th of 486
this lab
most citedleast cited
Career totals from OpenAlex, compared against labs that share a department. A department spans everything from one paper to tens of thousands, so bar heights are on a log scale — otherwise the largest lab would flatten every other bar to a line.