Rutgers University-New Brunswick · Microbiology & ImmunologyBacterial Transformation Research Lab
David A Dubnau · New Brunswick, New Jersey
Research Focus
David A Dubnau's lab at Rutgers Biomedical and Health Sciences focuses on understanding how bacteria can take up DNA from their environment, a process that can lead to antibiotic resistance and other traits. The lab studies the detailed mechanisms involved in this transformation process using the bacterium Bacillus subtilis as a model system. Researchers employ a variety of biochemical and biophysical methods to investigate the proteins and processes that facilitate this uptake and integration of DNA into bacterial genomes.
bacterial transformationantibiotic resistancegene transferBacillus subtilisDNA uptakerecombinationcell biologybiochemistrymolecular biologysingle-molecule biophysics
Research Aims
5AIM 01Investigate the mechanisms of DNA uptake in bacteria.
AIM 02Explore the role of ComFA and ComFC in DNA translocation.
AIM 03Study the CoiA protein's function in DNA recombination.
AIM 04Test the hypothesis of ComEA's role in DNA condensation and transport.
AIM 05Elucidate the steps involved in bacterial transformation.
Skills the Lab Values
cell culturegenetic manipulationbiochemical assaysstructural biology techniquessingle-molecule techniquesmicroscopyprotein purificationX-ray crystallographybiophysical analysismolecular cloning
Output against the department
Publications212top 30% of dept.147th of 486
this lab
most publishedleast published
Citations19ktop 20% of dept.97th of 486
this lab
most citedleast cited
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