University of Washington-Seattle Campus · BiochemistryUbiquitylation and Protein Regulation Lab
Rachel E Klevit · Seattle, Washington
Research Focus
Professor Rachel Klevit's lab at the University of Washington studies how proteins are modified by a process called ubiquitylation, which affects their function and stability in cells. This research is important for understanding various human diseases, including cancer, as the modification of proteins can influence cellular processes such as growth and stress responses. The lab focuses on the proteins involved in this modification process and aims to uncover new therapeutic targets to address these diseases.
ubiquitylationE3 ligasesprotein modificationcellular regulationbiochemical reactionshuman diseasescancer researchtranslational sciencestructural biology
Research Aims
5AIM 01Investigate the mechanisms of E2 and E3 enzymes involved in protein ubiquitylation.
AIM 02Characterize uncharacterized E2 and RBR E3 members and their substrates.
AIM 03Explore the relationships between mono-ubiquitylation and protein structure.
AIM 04Determine the impact of ubiquitin modifications on cellular processes.
AIM 05Develop strategies for therapeutic targeting of the ubiquitylation machinery.
Skills the Lab Values
protein purificationenzyme assayscrystallographycell culturemolecular cloningwestern blottingRNA extractionsite-directed mutagenesisbiophysical characterization
Output against the department
Publications296top 22% of dept.87th of 402
this lab
most publishedleast published
Citations18ktop 24% of dept.96th of 402
this lab
most citedleast cited
Career totals from OpenAlex, compared against labs that share a department. A department spans everything from one paper to tens of thousands, so bar heights are on a log scale — otherwise the largest lab would flatten every other bar to a line.