University of Toledo · ChemistryFlavin Halogenase Research Unit
John J. Bellizzi · Toledo, Ohio
Research Focus
Dr. John J. Bellizzi's lab focuses on exploring novel enzymes produced by bacteria and archaea, specifically flavin-dependent halogenases. These enzymes can attach halogen atoms to aromatic compounds, which are key components in pharmaceuticals and agrochemicals. The lab seeks to better understand how these enzymes work and how they can be used in green chemistry to create environmentally friendly chemical products.
halogenasesgreen chemistryenzyme characterizationhuman microbiomebiocatalystspharmaceutical synthesismetagenomicssubstrate specificitycrystallography
Research Aims
3AIM 01Identify and characterize novel flavin-dependent halogenases from the human microbiome and archaea.
AIM 02Investigate the substrate scope of known halogenases to identify new reaction products.
AIM 03Develop real-time assays for halogenase activity to facilitate high-throughput screening.
Skills the Lab Values
enzyme purificationsubstrate binding analysisprotein crystallizationhigh-pressure liquid chromatographyfluorescence spectroscopymetagenomic analysisstructural biologybioinformaticsmolecular cloning
Output against the department
Publications19bottom 3% of dept.210th of 216
this lab
most publishedleast published
Citations424bottom 4% of dept.208th of 216
this lab
most citedleast cited
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