University of Pennsylvania · PharmacologyAlcohol and Neuronal Response Unit
James H Eberwine · Philadelphia, Pennsylvania
Research Focus
Dr. James Eberwine's lab at the University of Pennsylvania studies how individual brain cells respond to alcohol, especially in the context of Alcohol Use Disorder (AUD). They are using advanced techniques to look at changes in gene activity and chromatin structure in single neurons and astrocytes after alcohol exposure. The goal is to understand the underlying biology of AUD and identify potential therapeutic targets by analyzing these cellular responses in their natural brain environment.
Alcohol Use Disorderneuronsastrocytesgene transcriptionchromatin structuremulti-omicscellular responsivenessethanolsingle-cell analysisbrain microenvironment
Research Aims
5AIM 01Assess the genomic effects of alcohol exposure on single neurons and astrocytes.
AIM 02Characterize the open-chromatin landscape and transcriptome in situ.
AIM 03Explore changes in gene activity linked to alcohol self-administration and withdrawal.
AIM 04Identify cellular mechanisms contributing to Alcohol Use Disorder.
AIM 05Develop techniques to analyze human brain tissue samples for AUD research.
Skills the Lab Values
RNA transcriptionchromatin analysissingle-cell sequencingimmunocytochemistrylight-activated oligonucleotide probesmouse modelsin situ hybridizationdata analysis with bioinformaticsbarcoding techniquesbehavioral phenotyping
Output against the department
Publications199top 44% of dept.204th of 462
this lab
most publishedleast published
Citations12ktop 31% of dept.145th of 462
this lab
most citedleast cited
Career totals from OpenAlex, compared against labs that share a department. A department spans everything from one paper to tens of thousands, so bar heights are on a log scale — otherwise the largest lab would flatten every other bar to a line.