University of Minnesota-Twin Cities · PharmacologySheedlo Toxin Lab
Michael J. Sheedlo · Minneapolis, Minnesota
Research Focus
Dr. Michael J. Sheedlo's lab focuses on understanding how the Clostridioides difficile Transferase (CDT) toxin works to infect host cells. They use advanced imaging techniques and biochemical methods to investigate the structure and function of CDT, aiming to find new ways to develop treatments for infections caused by this bacterium. By exploring how CDT interacts with cells, the lab seeks to uncover important details that could lead to better therapies for combating C. difficile infections.
Clostridioides difficiletoxin mechanismscryogenic electron microscopybiochemical assayshost-pathogen interactionstreatment developmentbacterial toxinstoxic megacolonpseudomembranous colitis
Research Aims
5AIM 01Define the mechanism of CDT intoxication in host cells.
AIM 02Investigate the regulation and assembly of CDT.
AIM 03Analyze the interaction of CDT and host cell membranes.
AIM 04Describe the activity of extrachromosomal CDT variants.
AIM 05Identify new therapeutic targets to inhibit CDT.
Skills the Lab Values
Cryogenic electron microscopybiochemical assayscell culturecellular biologyprotein analysisbiophysics techniquestoxicity assaysstructural biologymolecular modeling
Output against the department
Publications57bottom 9% of dept.419th of 462
this lab
most publishedleast published
Citations1.2kbottom 5% of dept.437th of 462
this lab
most citedleast cited
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