Oregon Health & Science University · Microbiology & ImmunologyUbiquitin Signaling and Bacterial Interactions Lab
Jonathan N Pruneda · Portland, Oregon
Research Focus
Dr. Jonathan N Pruneda’s lab focuses on understanding how bacteria interact with the immune system by manipulating a protein signaling system known as ubiquitin. The lab specifically investigates a less-studied form of ubiquitin signaling called Lys6 polyubiquitin, looking at how bacterial pathogens use this to evade immune responses and affect protein degradation in host cells. This research could reveal new strategies for combating bacterial infections and developing therapeutics.
ubiquitin signalingbacterial pathogenesisimmune evasionEscherichia coliLegionella pneumophilapost-translational modificationbacterial virulencedeubiquitinasesE3 ligases
Research Aims
4AIM 01Investigate how bacterial E3 ligases manipulate Lys6 polyubiquitin signaling.
AIM 02Characterize the molecular basis for Lys6 polyubiquitin specificity.
AIM 03Identify the role of bacterial deubiquitinases during infection.
AIM 04Explore the evolutionary benefits of atypical ubiquitin signals in host-pathogen interactions.
Skills the Lab Values
molecular cloningcell cultureprotein purificationWestern blottingmass spectrometryimmunofluorescence microscopypathogen infection modelsgenetic manipulation of bacteriabiochemical assays
Output against the department
Publications99bottom 34% of dept.320th of 486
this lab
most publishedleast published
Citations4.0kbottom 30% of dept.341st of 486
this lab
most citedleast cited
Career totals from OpenAlex, compared against labs that share a department. A department spans everything from one paper to tens of thousands, so bar heights are on a log scale — otherwise the largest lab would flatten every other bar to a line.